Patch amperometry: high-resolution measurements of single-vesicle fusion and release
نویسندگان
چکیده
منابع مشابه
Relationship between fusion pore opening and release during mast cell exocytosis studied with patch amperometry.
We have studied exocytosis in rat peritoneal mast cells by cell-attached patch amperometry. Step increases in capacitance were accompanied by typical amperometric spikes due to the release of 5-hydroxytryptamine (serotonin), indicating exocytosis of typical mast cell granules. We have measured the time course of fusion pore expansion, and correlated it with release from the granule matrix. The ...
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During exocytosis, vesicles in secretory cells fuse with the cellular membrane and release their contents in a Ca2+-dependent process. Release occurs initially through a fusion pore, and its rate is limited by the dissociation of the matrix-associated contents. To determine whether this dissociation is promoted by osmotic forces, we have examined the effects of elevated osmotic pressure on rele...
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Membrane fusion is a vital process of life involved, for example, in cellular secretion via exocytosis, signaling between nerve cells, and virus infection. In both the life sciences and bioengineering, controlled membrane fusion has many possible applications, such as drug delivery, gene transfer, chemical microreactors, or synthesis of nanomaterials. Until now, the fusion dynamics has been elu...
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Many cell types, including neurons, chromaffin cells of the adrenal adrenal gland and mast cells, release transmitter molecules stored in ‘vesicles’ by a process called exocytosis. In this process, an intracellular vesicle loaded with molecules to be secreted, fuses with the cell membrane, forming an initial opening called a fusion pore, and emits its contents into the extracellular space. The ...
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ژورنال
عنوان ژورنال: Nature Methods
سال: 2005
ISSN: 1548-7091,1548-7105
DOI: 10.1038/nmeth0905-699